Dr. Linda Roberts, Department Chair  

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Education

University of Vermont, BA Biology, 1984

Washington State University, PhD Biochemistry and Biophysics, 1990

Office:  SQU 506

Phone: (916) 278-6684

Email: robertslm@csus.edu

 




Home page

http://www.csus.edu/indiv/r/robertsl/

Courses

Chem. 5 - Chemistry for Nurses
Chem. 6B - Introduction to Organic and Biological Chemistry
Chem. 106 - Chemical Concepts
Chem. 161 - General Biochemistry
Chem. 160A - Stucture and Function of Biological Molecules
Chem. 160B - Metabolism and Regulation of Biological Systems
Chem. 162 - General Biochemistry Laboratory
Chem. 164 - Advanced Biochemistry Laboratory

Research:

Apolipoprotein A-I is the major protein component of high density lipoprotein (HDL, the "good" cholesterol). It plays an important role in lowering the risk for coronary artery disease. We are exploring the structural features of human and non-human apolipoprotein in order to understand its lipid-binding mechanisms.  We use the tools of molecular biology to create specific mutations and evaluate the effect of the mutations using a variety of biophysical methods.

For current students and their projects, click here .

Recent Publications and Presentations:

 PUBLICATIONS (*Denotes undergraduate author, + denotes MS student author)

  • Petrlova, J., +Duong, T., +Cochran, M., Axelsson, A., Morgelin, M., Roberts, L.M., Lagerstedt, J.O.  The Fibrillogenic L178H Variant of Apolipoprotein A-I Forms Helical Fibrils.  2012  J. Lipid Res. 53, 390-398.
  • Lagerstedt, J.O., Cavigiolio, G., Roberts, L.M., Hong, H.S., Jin, L.W., Fitzgerald, P.G., Oda, M.N., Voss, J.C.  Mapping the Structural Transition in an Amyloidogenic Apolipoprotein A-I.  2007.  Biochemistry 46, 9693-9699.
  • Roberts, L.M.  Developing Experimental Design and Trouble-shooting Skills in an Advanced Biochemistry Laboratory.  2001. Biochemistry and Molecular Biology Education 29, 10-15.
  • Rogers, D.P., Roberts, L.M., Lebowitz, J., Anantharamaiah, G.M., Engler, J.A., Brouillete, C.G.  The Lipid-Free Structure of Apolipoprotein A-I: Effects of Amino-Terminal Deletions. 1998. Biochemistry 37, 11714-11725 .
  • Rogers, D.P., Roberts, L.M., Lebowitz, J., Engler, J.A., Brouillette, C.G.Structural Analysis of Apolipoprotein A-I: Effect of Amino- and Carboxyl-Terminal Deletions on the Lipid-Free Structure.  1998. Biochemistry 37, 945-955.
  • Roberts, L.M., Ray, M.J.,Shih, T.-W., *Hayden, E., *Reader, M.M., Brouillette, C.G.  Structural Analysis of Apolipoprotein A-I: Limited Proteolysis of Methionine-Reduced and -   Oxidized Lipid-Free and Lipid-Bound Human Apo A-I.  1997. Biochemistry 36, 7615-7624.
  • Rogers, D.P., Brouillette, C.G., Engler, J.A., Tendian, S.W., Roberts, L.M., Mishra, V., Anantharamaiah, G.M., Lund-Katz, S., Phillips, M.C., Ray, M.J.  Truncation of the Amino Terminus of Human Apolipoprotein A-I Substantially Alters Only the Lipid-Free Conformation.  1997. Biochemistry 36 , 288-300.
  • Roberts, L.M., Dunker, A.K.  Structural Changes Accompanying Chloroform-Induced Contraction of the Filamentous Phage fd.  1993. Biochemistry 32 , 10479-10488.
  • Dunker, A.K., Ensign, L.D., Arnold, G.E., Roberts, L.M.  A Model for fd Phage Penetration and Assembly.  1991. FEBS Letters 292, 271-274.
  • Dunker, A.K., Ensign, L.D., Arnold, G.E., Roberts, L.M.  Proposed Molten Globule Intermediate in fd Phage Penetration and Assembly. 1991. FEBS Letters 292, 275-278.

Presentations (since 1996) (*Denotes CSUS undergraduate authors, #denotes master's students)

Assessing the Effect of Neutral Phospholipid on Fibril Formation in L178H Apolipoprotein A-I.  Truong, H*., Roberts, L.M.  2014 Annual CSUPERB meeting, Santa Clara, CA.

Construction and expression of a fibril-forming mutant of Apolipoprotein A-I.  Rutaganira, A*., Roberts, L.M.  2014 Northern California Undergraduate ACS Meeting.

The cationic detergent DTAC does not influence fibril formation in fibrillogenic human apolipoprotein A-I.  Hawkins, S*., Knudsen*, A., Roberts, L.M.  2013 Annual CSUPERB meeting, Anaheim, CA.

Analysis of a lipid-bound fibrillogenic variant of human apolipoprotein A-I.  Leyva-Castro, A#., Roberts, L.M.  2013 Annual CSUPERB meeting, Anaheim, CA.

Optimizing the yield of human Apolipoprotein A-I from a bacterial expression system.  Kongipanickul, C*., Roberts, L.M.  2013 Northern California Undergraduate ACS Meeting.

Cholate inhibits fibril formation in ApoA-I.  McCray, W*., Truong, H*., Roberts, L.M.  2013 Northern California Undergraduate ACS Meeting.

The Effect of DTAC on WT and L178H Apolipoprotein A-I Fibril Formation.  Knudsen, A*., Hawkins, S*., Roberts, L.M.   2013 Northern California Undergraduate ACS Meeting.

Differential effect of detergent suggests different aggregated structures in wild-type vs amyloid Apolipoprotein A-I.  Bal, Y*., Ngyuen, H*, Roberts, L.M.  2012 Annual CSUPERB meeting, Oakland, CA.

Decreased stability in the amyloidogenic L178H variant of human apolipoprotein A-I.  Duong, T#., Lagerstedt, J.O., Roberts, L.M.  2012 Annual CSUPERB meeting, Oakland, CA.

Decreased pH and Elevated Temperature Cause Greater Aggregation of the L178H Amyloidogenic Variant Compared to WT Apolipoprotein A-I.  Bustamante-Hawkins, S*., Musgrove, L*., Roberts, L.M.  2012 Annual CSUPERB meeting, Oakland, CA.

The amyloidogenic L178H mutation in apolipoprotein A-I decreases the protein stability.  Duong, T#., Roberts, L.M. 2011 Annual CSUPERB meeting, Anaheim, CA.

Structural properties of amyloidogenic apoA-I mutant L178H.  Annika Axelsson, Megan Cochran#, Trang Duong#, Miral Chokshi, John C Voss, Linda M Roberts, Jens O Lagerstedt.   FEBS Letters Volume 277,  Supplement 1, A3.63. 2010

The amyloid apolipoprotein variant L178H does not bind the amyloid-detecting dye ThT".  Angela Monterrubio* and Linda M. Roberts.  2010 Northern California Undergraduate ACS meeting.

Analysis of the formation of insoluble aggregates by the amyloid apolipoprotein variant L178H and WT.  Mustafa Safi,* Mai Thao,# and Linda M. Roberts.  2010 Northern California Undergraduate ACS meeting.

Limited proteolysis of the amyloid apolipoprotein variant L178H in the lipid-bound state.  Adam Floyd* and Linda Roberts.  2010 Northern California Undergraduate ACS meeting.

The detection of amyloid fibril formation in a mutant apolipoprotein using the amyloid-detecting dye ThT.  Monterrubio*, A., Roberts, L.M.  2009 ACS Undergraduate Meeting, Moraga, CA.

Limited Proteolysis of Amyloidogenic Lipid-Bound Apolipoprotein A-I Implicates Lipid-Free Protein in Amyloid Formation.  S. Hilt*, L.M. Roberts, J.O. Lagerstedt, M.N. Oda, J.C. Voss.  22nd Symposium of the Protein Society, San Diego, CA.  (Silvia Hilt won an undergraduate poster award.) 2008

The Structural Transition Between Lipid-free and Lipid-bound States of Apolipoprotein A-I is Controlled by Residues 1-23.  J. Case*, S. R. Randall, T. Postiglione*, J.O. Lagerstedt, L.M. Roberts.  2008 CSUPERB Annual Meeting, Oakland, CA.

Limited Proteolysis of Amyloidogenic Lipid-Bound Apolipoprotein A-I Implicates Lipid-Free Protein in Amyloid Formation.  S. Hilt*, L.M. Roberts, J.O. Lagerstedt, J.C. Voss.   2008 CSUPERB Annual Meeting, Oakland, CA.

Developing Experimental Design and Trouble-shooting Skills in an Advanced Biochemistry Lab.  Roberts, L.M., McReynolds, K., McCarthy, M.  Protein Society Meeting.  Boston, MA.  2005.

Lipid-free Structure and Lipid-Binding etc. etc. Roberts, L.M., Shasky, J.M, Tendian, S.W.  Protein Society Meeting. Boston, MA. 2005.

Infectivity of Phage fd I-forms and S-forms to E. Coli Cells.  Smith, M., Roberts, L.M.  CSUPERB Annual Biotechnology Symposium, Pomona, CA.  2002. 

Insertion Competency of Filamentous Phage fd.  Sean, P., Robertson, S.A., Wells, J.A., Contreras, A., Roberts, L.M.  Protein Society Meeting, Philadelphia, PA.  2001. 

Characterization of High Density Lipoproteins Containing Apolipoprotein A-I Deletion Mutants.  Smyth, N., Almaraz, R., Galvan-Rueckert, F., Engler, J.A., Brouillette, C.G., Roberts, L.M.  ACS National Meeting, San Francisco, CA.  2000. 

An Insertion Competent Conformation of the Filamentous Phage fd:  Fusion of I-forms With Lipid Vesicles.  Robertson, S.A., Roberts, L.M.  ACS National Meeting, San Francisco, CA.  2000. 

Localization of Bacteriophage DNA Following Fusion of Phage with Lipid Vesicles.  Wells, J., Contreras, A., Mercer, V., Roberts, L.M.  Undergraduate ACS Meeting, Hayward, CA.  2000. 

Chemical Modification of Tryptophan and Tyrosine Residues: Effect on Apolipoprotein A-I Conformation. Slack, D., McGann, S.J., Moore, L., Greene, S., Mymka, M., Woolsey, C., Roberts, L.M. 9th Annual ACS Undergraduate Research Symposium , U.C. Santa Cruz.  1997. 

Apolipoprotein A-I: A Naturally Occurring Molten Globule. Roberts, L.M., Rogers, D.P., Engler, J.A., Brouillette, C.G. Protein Science 5, supp 1:79.  1996.