1. Is there an advantage to
running a 2D HPLC instrument with two pumps vs. a set up with a single pump? If
yes, what are the advantages?
A: Yes. 2D HPLC instrument with two pumps allows for two different types of mobile phase to be used in separation, if necessary.
2. Why is the 2D HPLC a more preferred method for analysis of large matrix samples?
A: 2D HPLC allows for a very large peak capacity as well as better separation of analytes within the sample.