Chris Peery

12/2/14

Chem 230

SPME-HPLC Homework Questions

1. What SPME technique adsorbs analytes from the air? From water?

2. Name two advantages and one disadvantage SPME has compared to other HPLC techniques.

3. If the coating on an SPME microfiber is 7 µm and it takes 5 minutes for an analyte to desorb, what is the diffusion coefficient of the analyte (in µm²/min)?

4. Name one difficulty inherent in the analysis of pesticides and how the SPME technique addresses this issue.