Questions:

1) Why is it advantageous to use zwitterionic stationary phase compared to other liquid chromatography stationary phase discussed in this class?  And why it might be disadvantageous?

2) Using the sulfobetaine stationary phase, would cations or anions be retained more strongly?

3) A chemist was analyzing oligomers of a protein using HILIC/CEX with an ELS detector. The detector showed the most response to the later eluting peaks. What can be said about the average size of oligomer the protein forms?

4) List three factors that can affect selective and retention using a zwitterionic column.