Updated: 22 March, 2007
 
  Bio 184 Practical 3

Enzyme Induction

 

Calculations
You are required to perform some calculations based on your own observed data...

Results:

Sample

A600 Reading

A420 Reading

1 (Glucose)

.950

.025

2 (Lactose)

.749

2.0

3 (Sucrose)

.289

.040

4 (Water)

.330

.071

 

Sample

# Cells/ml x 10 E8
(based on A600 reading)

Units enzyme activity/ml
(based on A420 reading)

Units enzyme activity /10 E8 cells

1 (Glucose)

.95

2.5

2.63

2 (Lactose)

.75

200

266.7

3 (Sucrose)

.29

4.0

13.8

4 (Water)

.33

7.1

21.52

You must look at the units of each column of the table to obtain a clue as how to perform the calculations.

 


 

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