Chemistry 240 Advanced Instrumentation Laboratory, Spring 2010

Instructor: Dr. Cynthia Kellen-Yuen

Office: SQU 424A phone: 278-3528 E-mail: ckyuen@csus.edu

Office hours: M 3-4 and T,R 9-10, or by appointment

Faculty website: http://www.csus.edu/indiv/k/kellen-yuenc

Class SacCT site: https://online.csus.edu

Course Description: Synthesis of compounds and application of modern separation techniques to determine structure and reactivity will be emphasized. Organic, inorganic and/or biological chemicals may be synthesized. Instruction methods that may be used include: HPLC, FT-IR, NMR, UV-vis, fluorescence, atomic absorption, mass spectrometry and cyclic voltammetry. Prerequisites: Chem 24, Chem 25, Chem 124 ( i.e. one year of undergraduate organic lecture and laboratory).

Chem 240 will focus on the application of the information gained in Chem 220—Spectrometric Identification of Compounds. This class will involve synthesis, purification and spectrometric identification of a variety of compounds. Students are expected to have mastered all of the basic skills of organic synthesis, since this class does not focus on teaching these techniques. Rather it is focused on the use of instrumental techniques to identify compounds. Students will be required to present the results of these studies in both oral Power Point presentations and in formal, written laboratory reports.

All reports must be done with a word processor, embedding spectra in the body of the text close to the point of discussion. All structures for reports or presentations must be drawn with ChemSketch, ChemDraw, ISIS Draw, or some other structure drawing program. An electron-pushing mechanism should be provided with all synthesis projects. Citations are expected, and must be formatted according to JACS guidelines. No handwritten reports or additions to the reports will be accepted.

Textbooks for This Course: Two textbooks are needed for Chemistry 240. The first one is the text you used for your undergraduate organic laboratory. This book will provide you with a review and guide as to how to do some of the standard operations in the laboratory. Be sure to review each technique as you do it again in this laboratory. If you have never performed a technique, thoroughly study the appropriate section in your old text.

The second text that is required for this course is the text used in the Chemistry 220 course: “Introduction to Spectroscopy,” 3^rd edition, by Pavia, et al.

An excellent book with tables of reference data is: “Spectrometric Identification of Organic Compounds,” 7th edition, by Silverstein, et al. This book is indispensable when trying to analyze IR, NMR, and mass spectral data. While this book is not required as a cost-saving measure, any student who plans a future project involving spectroscopic analysis will find this book to be indispensible.

On the SacCT website you will also find an electronic version of a “lab manual” which offers helpful reminders on techniques you were supposed to have mastered already. It also provides some review on NMR analysis and some very helpful reference tables (NMR and solvent data). Most importantly, you will find instructions on how to run the instruments which we will be using in this course. Download these instructions and bring them with you to lab to make sure you know how to run the instruments.

Items to Purchase for This Course

1. Bound laboratory notebook. You will be required to keep notes in this book and will turn it in for grading to your instructor at the end of the semester.

2. Goggles. These are required for protection of your eyes. The rule is: “No Goggles, No Work in the Laboratory!” This is an absolute, and any student caught repeatedly working in lab without goggles will be dropped from the course—this rule exists whether or not the instructor is in the room at the time. NO CONTACTS MAY BE WORN IN THE LAB AT ANY TIME.

3. Gloves. Purchase a box of nitrile gloves. Alternatively, you may purchase a pair of blue rubber gloves from a grocery store. Be sure to get the correct size; a larger size is easy to get on and off. Gloves should always be worn when you pour liquids from one container to another. This is for your protection, but is optional.

Lab Notebook You will keep a bound lab notebook which will contain the following:

1. Date of work as it is performed

2. Reaction/Experiment being performed

3. Pertinent physical constants of the organic starting materials and potential products: mp of solids and bp of liquids.

4. An outline of what you are going to do.

5. Any potential hazards such as: fire, toxicity, etc. See Dangerous Properties of Industrial Materials edited by Sax or any standard MSDS for a listing of hazards of known compounds.

6. A record of what you actually did: masses of reactants, volumes of liquids, concentrations of solutions, actual procedure (not what the experimental procedure told you to do), mass and yield of product, mp, bp, etc. Additionally list what analyses you have run on any given compound, observations and data obtained, as well as a brief conclusion about your overall findings. This information, along with the spectral and chromatographic data, will be used in writing the lab report.

Approximate Point Distribution for the Final Semester Grade:

	Points
Spectral problem set	60
Experiment 1—Power Point presentation	100
Experiment 2—Formal lab report	100
Experiment 3—Formal lab report	100
Experiment 4—Formal lab report	100
Final Project—Power Point presentation	100
Exam	100
Lab book / Instructor evaluation	40
Total	700

Items to Purchase for This Course

Grading will follow the percentages of the total points earned:

100-90% A, 89-80% B, 79-70% C, 69-60% D, below 60% F